This PhD project focuses on the combination of imaging approaches which allow to bridge the gap between different resolutions, from the nano to the macro scale, and approaches looking for already known analytes (targeted analysis) or visualizes structures by highlighting the distributions of yet unknowns (untargeted). With a strong emphasis on imaging mass spectrometry, we will look for lipid, metabolite, (glycol)protein changes in fixed and unfixed skin equivalents. New molecular probes will be developed for in vivo labelling not only to increase sensitivity but also specificity of detection to innovatively combine imaging techniques like mass spectrometry with Raman/IR, high-resolution fluorescence microscopy, atomic force microscopy, electron microscopy and other MS-based methods (SIMS, LAICP) to ultimately get a better understanding of biological events relevant for UV induced skin aging.