Using the single-molecule fluorescence microscopy modality “TOCCSL” and flux assays, the subunit stoichiometry and function of the norepinephrine transporter (NET) was investigated. NET was found to form monomers (~60%) and dimers (~40%) at the plasma membrane. The average oligomeric state was found to decrease upon PIP₂ depletion.

Authors:

Dino Luethi ^1 2, Julian Maier ¹, Deborah Rudin ¹, Dániel Szöllősi ¹, Thomas J F Angenoorth ¹, Stevan Stankovic ¹, Matthias Schittmayer ³, Isabella Burger ³, Jae-Won Yang ¹, Kathrin Jaentsch ¹, Marion Holy ¹, Anand Kant Das ^2 4, Mario Brameshuber ², Gisela Andrea Camacho-Hernandez ⁵, Andrea Casiraghi ^5 6, Amy Hauck Newman ⁵, Oliver Kudlacek ¹, Ruth Birner-Gruenberger ^3 7, Thomas Stockner ¹, Gerhard J Schütz ⁸, Harald H Sitte ⁹

Reference:

Communications Biology volume 5, Article number: 1259 (2022)