Phosphatidylinositol 4,5-bisphosphate (PIP2) facilitates norepinephrine transporter dimerization and modulates substrate efflux
Using the single-molecule fluorescence microscopy modality “TOCCSL” and flux assays, the subunit stoichiometry and function of the norepinephrine transporter (NET) was investigated. NET was found to form monomers (~60%) and dimers (~40%) at the plasma membrane. The average oligomeric state was found to decrease upon PIP2 depletion.
Communications Biology volume 5, Article number: 1259 (2022)