A customized micropatterning approach combined with total internal reflection fluorescence microscopy was used to quantify ligand-induced FGFR3 receptor activation at the live cell membrane. Activation was recorded via the recruitment of the downstream signal transducer growth factor receptor-bound 2 (GRB2) to FGFR3 micropatterns.


Ingrid Hartl 1Veronika Brumovska 2Yasmin Striedner 1Atena Yasari 1Gerhard J Schütz 2Eva Sevcsik 3Irene Tiemann-Boege 4


Journal of Biological Chemistry, 2023. DOI:https://doi.org/10.1016/j.jbc.2022.102832



Check out our Instagram profile and follow us for bursts of inspiration.