A customized micropatterning approach combined with total internal reflection fluorescence microscopy was used to quantify ligand-induced FGFR3 receptor activation at the live cell membrane. Activation was recorded via the recruitment of the downstream signal transducer growth factor receptor-bound 2 (GRB2) to FGFR3 micropatterns.

Authors:

Ingrid Hartl ¹, Veronika Brumovska ², Yasmin Striedner ¹, Atena Yasari ¹, Gerhard J Schütz ², Eva Sevcsik ³, Irene Tiemann-Boege ⁴

Reference:

Journal of Biological Chemistry, 2023. DOI:https://doi.org/10.1016/j.jbc.2022.102832

https://www.jbc.org/article/S0021-9258(22)01275-3/fulltext