TIME RESOLVED SPECTROSCOPY
Time-resolved fluorescence microscopy is a powerful technique for studying single molecule interactions. It allows for high-resolution imaging of single molecule localizations and diffusion, particularly on biomembranes. Our facilities also offer fluorescence lifetime mapping and fluorescence anisotropy imaging to provide more information about the dynamics and interactions of molecules. Time resolved spectroscopy observes the change in eigenstates with respect to time. To obtain useful data, the spectroscopy must be suited to the time scale of the process of interest. For example, the extremely short molecule interactions can be measured in picoseconds, and our equipment is capable of capturing such fast events.
Application
– Single molecule diffusion studies
– FRAP (fluorescence recovery after photobleaching)
– FLIM / FRET (fluorescence lifetime imaging microscopy)
– Cell biology
– Bioavailability assays
