Transmission Electron Microscopy (TEM) is an imaging tool that relies on the physical principles of electrons to shape and form an electron beam using electromagnetic lenses within a vacuum. This technique achieves nanometer resolution on well-prepared biological specimens. Sample preparation involves fixation and staining. In contrast, cryo-TEM rapidly freezes samples without chemical fixation or heavy metal staining to visualize samples ranging from proteins to cells in their close-to-native state.
– Tissue ultrastructure
– Cell interactions
– General cell and tissue biology