TRANSMISSION ELECTRON MICROSCOPY (TEM)
Transmission Electron Microscopy (TEM) is an imaging tool that relies on the physical principles of electrons to shape and form an electron beam using electromagnetic lenses within a vacuum. This technique achieves nanometer resolution on well-prepared biological specimens. Sample preparation involves fixation and staining. In contrast, cryo-TEM rapidly freezes samples without chemical fixation or heavy metal staining to visualize samples ranging from proteins to cells in their close-to-native state.
Application
– Tissue ultrastructure
– Cell interactions
– General cell and tissue biology
– Cryo-TEM
