Using the single-molecule fluorescence microscopy modality “TOCCSL” and flux assays, the subunit stoichiometry and function of the norepinephrine transporter (NET) was investigated. NET was found to form monomers (~60%) and dimers (~40%) at the plasma membrane. The average oligomeric state was found to decrease upon PIP2 depletion. Authors: Dino Luethi 1 2, Julian Maier 1, Deborah......
Careful phenotype analysis of genetically altered mouse embryos/fetuses is vital for deciphering the function of pre- and perinatally lethal genes. Usually this involves comparing the anatomy of mutants with that of wild types of identical developmental stages. Detailed three dimensional information on regular cranial nerve (CN) anatomy of prenatal mice......
Approximately one-third of randomly produced knockout mouse lines produce homozygous offspring, which fail to survive the perinatal period. The majority of these die around or after embryonic day (E)14.5, presumably from cardiovascular insufficiency. For diagnosing structural abnormalities underlying death and diseases and for researching gene function, the phenotype of these......