21 November 2024
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18 November 2024This work introduces advanced microDECT imaging protocols that provide unprecedented visualization and analysis of microvasculature in intact tissue, enhancing our understanding of vascular structures in relation to soft and hard tissue morphology. By enabling the effective separation of vasculature from skeletal tissues and facilitating the integration of multiple staining techniques,......
19 August 2024Austrian BioImaging/CMI is proud to highlight new research by our member, Kareem Elsayad, from the Medical University of Vienna, which was recently published in the prestigious journal PNAS. In this work, Kareem and his team demonstrate that measuring blood plasma viscosity using Brillouin light scattering (BLS) spectroscopy is a valuable method......
- 25 April 2024
Oliver Renaud 1, Nathalie Aulner 2, Audrey Salles 2, Nadia Halidi 3, Maia Brunstein 4, Adeline Mallet 5, Karin Aumayr 6 7, Stefan Terjung 8, Daniel Levy 1, Saskia Lippens 9, Jean-Marc Verbavatz 10, Thomas Heuser 11, Rachel Santarella-Mellwig 12, Jean-Yves Tinevez 13, Tatiana Woller 14 15, Alexander Botzki 16, Christopher Cawthorne 17; Core4Life Consortium; Sebastian Munck 15 18 Abstract: Modern life science research is a collaborative effort. Few research groups can single-handedly support the necessary equipment, expertise and personnel needed for the ever-expanding portfolio of technologies that are......
19 January 2024Kareem Elsayad and his research team at the Center for Anatomy and Cell Biology, a part of the Medical Imaging Cluster at MedUni Vienna, have made significant strides in addressing complex questions related to cellular mechanics. Their innovative technique utilizes Brillouin Light Scattering to image intricate mechanical features within the......
24 April 2023Determination of the oligomerization state of plasma membrane proteins and lipids is key to understanding cellular signaling and function. TOCCSL (thinning out clusters while conserving stoichiometry of labeling)—an imaging modality based on single-molecule fluorescence microscopy—allows for measuring the degree of homo- or hetero-oligomerization of membrane constituents even at expression levels......
30 March 2023A customized micropatterning approach combined with total internal reflection fluorescence microscopy was used to quantify ligand-induced FGFR3 receptor activation at the live cell membrane. Activation was recorded via the recruitment of the downstream signal transducer growth factor receptor-bound 2 (GRB2) to FGFR3 micropatterns. Authors: Ingrid Hartl 1, Veronika Brumovska 2, Yasmin Striedner 1, Atena Yasari 1, Gerhard J......
19 November 2022Using the single-molecule fluorescence microscopy modality “TOCCSL” and flux assays, the subunit stoichiometry and function of the norepinephrine transporter (NET) was investigated. NET was found to form monomers (~60%) and dimers (~40%) at the plasma membrane. The average oligomeric state was found to decrease upon PIP2 depletion. Authors: Dino Luethi 1 2, Julian Maier 1, Deborah......
10 November 2022Careful phenotype analysis of genetically altered mouse embryos/fetuses is vital for deciphering the function of pre- and perinatally lethal genes. Usually this involves comparing the anatomy of mutants with that of wild types of identical developmental stages. Detailed three dimensional information on regular cranial nerve (CN) anatomy of prenatal mice......
- 1 April 2022
Magdalena C Schneider and Gerhard J Schütz describe how significance testing provides an important and valid addendum to the toolbox of quantitative (single molecule) biology. For two selected applications of single molecule microscopy – Nanoclustering in single molecule localization microscopy (SMLM) and single molecule tracking – they introduce how a single overall p-value......